Neutralization of primary human immunodeficiency virus type 1 isolates by the broadly reactive anti-V3 monoclonal antibody, 447-52D—Conley 1994

HIV-1 gp120 external glycoprotein V3 is bound by human monoclonal antibody 447-52D. The V3 domain's central GPxR sequence is needed for binding. The antibody efficiently neutralizes HIV-1 variants that are adapted to replication in T-lymphoid cell lines and express this sequence motif (M. K. Gorny, A. J. Conley, S. Karwowska, A. Buchbinder, J.-Y. Xu, E. A. Emini, S. Koenig, and S. Zolla-Pazner, J. Virol. 66:7538-7542, 1992). In this investigation, 447-52D was shown to neutralize primary HIV-1 clinical isolates. The antibody neutralized binding sequence-expressing isolates again. We found that subtype (clade) B virus isolates express this determinant. Thus, 447-52D may aid immunotherapy and prevention. An antibody can neutralize initial HIV-1 isolates, the study showed.

Conley, A. J., Gorny, M. K., Kessler, J. A., 2nd, Boots, L. J., Ossorio-Castro, M., Koenig, S., Lineberger, D. W., Emini, E. A., Williams, C., & Zolla-Pazner, S. (1994). Neutralization of primary human immunodeficiency virus type 1 isolates by the broadly reactive anti-V3 monoclonal antibody, 447-52D. Journal of virology, 68(11), 6994–7000. https://doi.org/10.1128/JVI.68.11.6994-7000.1994