A High-Throughput Assay for Circulating Antibodies Directed Against the S Protein of Severe Acute Respiratory Syndrome Coronavirus 2—Weiss 2020

By September 2020, SARS-CoV-2 had infected over 24 million people worldwide. Diagnostic polymerase chain reaction assays require high-throughput, fast serologic procedures. Human serum and plasma were tested for COVID-19-specific antibodies using a Luminex binding assay. Infected specimens showed a wide range of serum/plasma antibody levels. All 25 tissues from 18 COVID-19 patients tested positive for trimeric spike and receptor-binding domain proteins. Both antigens were absent from the 13 uninfected specimens. The antibody levels of COVID-19-infected and -uninfected specimens differed significantly (P <.001). Accurate, 2.5-hour, 5-ng-antigen high-throughput antibody assay.

Weiss, S., Klingler, J., Hioe, C., Amanat, F., Baine, I., Arinsburg, S., Kojic, E. M., Stoever, J., Liu, S. T. H., Jurczyszak, D., Bermudez-Gonzalez, M., Simon, V., Krammer, F., & Zolla-Pazner, S. (2020). A High-Throughput Assay for Circulating Antibodies Directed Against the S Protein of Severe Acute Respiratory Syndrome Coronavirus 2. The Journal of infectious diseases, 222(10), 1629–1634. https://doi.org/10.1093/infdis/jiaa531