Recent advances in the characterization of HIV-1 neutralization assays for standardized evaluation of the antibody response to infection and vaccination—Polonis 2008

Neutralizing antibodies are again being considered for a worldwide AIDS vaccine. Vaccine-induced antibody performance in neutralization testing may be a "gatekeeper" for HIV-1 subunit vaccine prioritization and advancement. Thus, a standardized platform for reliable neutralizing antibody measurement has garnered attention. We examine current knowledge of antibody performance in a primary cell neutralization experiment and the more standardized TZM-bl reporter cell line assay. Parallel examination with both platforms should be considered until immunological correlates of protection are better understood due to recent variations (see accompanying article) in results from these two neutralization formats.

Polonis, V. R., Brown, B. K., Rosa Borges, A., Zolla-Pazner, S., Dimitrov, D. S., Zhang, M. Y., Barnett, S. W., Ruprecht, R. M., Scarlatti, G., Fenyö, E. M., Montefiori, D. C., McCutchan, F. E., & Michael, N. L. (2008). Recent advances in the characterization of HIV-1 neutralization assays for standardized evaluation of the antibody response to infection and vaccination. Virology, 375(2), 315–320. https://doi.org/10.1016/j.virol.2008.02.007